| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2006579 | Peptides | 2011 | 9 Pages |
The aim of the present study was to evaluate in hypothalamus and hindbrain of rainbow trout in vitro the effect of leptin treatment on glucosensing capacity and the expression of orexigenic and anorexigenic peptides involved in the control of food intake. In a first experiment, the response of parameters involved in glucosensing (GK, PK and GSase activities; GK expression and glucose; glycogen and DHAP levels) and the expression of orexigenic (NPY) and anorexigenic (POMC, CART, CRF) peptides was assessed in hypothalami and hindbrain incubated for 1 h with 2, 4 or 8 mM d-glucose alone (controls) or with 10 nM leptin, or with 10 nM leptin plus inhibitors of leptin signaling pathways (50 nM wortmannin and 500 nM AG490). Leptin treatment increased levels in parameters involved in glucosensing. Leptin treatment decreased NPY mRNA levels in hypothalamus without affecting the expression of the other peptides assessed. Leptin effects were reverted in the presence of inhibitors for all parameters assessed suggesting the involvement of JAK/STAT and IRS-PI(3)K pathways. In a second experiment, we observed time-dependent (1–3 h) and dose (10, 20 and 50 nM)- effects of leptin treatment in decreasing NPY mRNA levels without affecting expression of the other peptides assessed. Considering the orexigenic action of NPY in fish, it seems that the anorexic effect of leptin can be mediated by reduced expression of NPY occurring in hypothalamus, and that change can be related to the activation of the glucosensing system occurring simultaneously.
Research highlights▶ Leptin treatment directly activates glucosensor system in hypothalamus and hindbrain of rainbow trout in a way dependent on glucose concentration. ▶ Leptin treatment inhibits NPY mRNA levels in hypothalamus without affecting POMC, CART and CRF mRNA levels. ▶ Leptin treatment does not affect NPY, POMC, CART and CRF mRNA levels in hindbrain. ▶ The anorexic effect of leptin in fish can be mediated by reduced expression of NPY in hypothalamus and may be related to activation of the glucosensing system.
