Article ID Journal Published Year Pages File Type
2006664 Peptides 2011 7 Pages PDF
Abstract

Vaccination against cholesteryl ester transfer protein (CETP) is proven to be effective for inhibiting atherosclerosis in animal models. In this study, the proteases-resistant intestinal trefoil factor (TFF3) was used as a molecular vehicle to construct chimeric TFF3 (cTFF3) containing CETP B cell epitope and tetanus toxin helper T cell epitope. It was found that cTFF3 still preserved a trefoil structure, and can resist proteases digestion in vitro. After oral immunization with cTFF3, the CETP-specific IgA and IgG could be found in intestine lavage fluid and serum, and the anti-CETP antibodies could inhibit partial CETP activity to increase high-density lipoprotein cholesterol, decrease low-density lipoprotein cholesterol, and inhibit atherosclerosis in animals. Therefore, TFF3 is a potential molecular vehicle for developing oral peptide vaccines. Our research highlights a novel strategy for developing oral peptide vaccines in the future.

Research highlights▶ The chimeric ITF containing CETP B cell epitope can form the correct trefoil structure. ▶ The chimeric ITF can resist the proteases digestion in vitro assay. ▶ The chimeric ITF can induce both of mucosal and systemic immune response in rabbits after oral administration. ▶ The anti-CETP antibodies induced by oral vaccination with cITF can inhibit CETP activity, increase HDL-C, decrease LDL-C and inhibit atherosclerosis in rabbits.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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