Article ID Journal Published Year Pages File Type
2007644 Peptides 2007 7 Pages PDF
Abstract

A 20-kDa protein with substantial N-terminal sequence homology to thaumatin-like proteins was isolated from ripe fruits of the emperor banana, Musa basjoo cv. ‘emperor banana’. The isolation procedure entailed (NH4)2SO4 precipitation, ion exchange chromatography on DEAE-cellulose, and affinity chromatography on Affi-gel blue gel. The thaumatin-like protein inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola. However, it did not affect the mitogenic response of murine splenocytes or [methyl-3H] thymidine incorporation by tumor cells. The activity of HIV-1 reverse transcriptase was slightly inhibited.

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