Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2007820 | Peptides | 2006 | 8 Pages |
A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.