Article ID Journal Published Year Pages File Type
20082 Journal of Bioscience and Bioengineering 2016 5 Pages PDF
Abstract

•ATPS extractive bioconversion composed by PEG and potassium phosphates solution.•The ATPS was optimized and ATPS extractive bioconversion was then applied.•Phase composition and phase concentration influenced the partition behavior of γ-CD.•The γ-CD concentration of 1.60 mg/mL with 19% concentration ratio was recovered after 1 h extractive bioconversion process.

Aqueous two-phase system (ATPS) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. Extractive bioconversion of gamma-cyclodextrin (γ-CD) from soluble starch with cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) enzyme derived from Bacillus cereus was evaluated using polyethylene glycol (PEG)/potassium phosphate based on ATPS. The optimum condition was attained in the ATPS constituted of 30.0% (w/w) PEG 3000 g/mol and 7.0% (w/w) potassium phosphate. A γ-CD concentration of 1.60 mg/mL with a 19% concentration ratio was recovered after 1 h bioconversion process. The γ-CD was mainly partitioned to the top phase (YT = 81.88%), with CGTase partitioning in the salt-rich bottom phase (KCGTase = 0.51). Repetitive batch processes of extractive bioconversion were successfully recycled three times, indicating that this is an environmental friendly and a cost saving technique for γ-CD production and purification.

Related Topics
Physical Sciences and Engineering Chemical Engineering Bioengineering
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