Article ID Journal Published Year Pages File Type
2008265 Peptides 2008 9 Pages PDF
Abstract

Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.

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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels
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