Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2008353 | Peptides | 2006 | 6 Pages |
Abstract
Two different RNAi methods were used to inhibit the expression of prohormone convertase 1 (PC1) in At-T20 cells. Transient transfection of double stranded RNA and stable expression of a vector expressing hairpin-loop RNA targeting PC1 reduced cholecystokinin (CCK) secretion from At-T20 cells. PC1 mRNA and protein were also decreased in the vector transfected cells. This treatment caused a shift in the forms of cholecystokinin (CCK) secreted, decreasing CCK 22 and increasing CCK 8. Stable expression of RNAi effectively decreased PC1 expression. The observed decrease in CCK seen with these RNAi treatments further supports a role for PC1 in CCK processing in these cells.
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Authors
Margery C. Beinfeld, Daesety Vishnuvardhan, Alissa Blum, Nicole Reynolds, Sanya Fannous, Kouki Kitagawa, James E. Marchand,