| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2008573 | Peptides | 2007 | 10 Pages |
Abstract
A short neuropeptide F (sNPF) precursor and a sNPF receptor (sNPFR) were characterized for the mosquito, Anopheles gambiae. The sNPFR was expressed in CHO-K1 cells, and it exhibited high affinity binding, IC50 â¼3-5Â nM, for specific sNPFs. sNPF1 potently inhibited forskolin-stimulated cAMP production by transfected cells, suggesting sNPFR acts via Gi/o. Transcripts for sNPF and sNPFR were present in all body regions of larvae, pupae, and adults, and immunoblots for sNPFR confirmed this distribution in females. Membranes from female heads and thoraces exhibited prototypical high affinity binding for radiolabeled sNPF, indicating sNPFR is a bona fide endogenous receptor.
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Authors
Stephen F. Garczynski, Joe W. Crim, Mark R. Brown,