Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
20100 | Journal of Bioscience and Bioengineering | 2016 | 4 Pages |
Abstract
We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Isaac Vizcaino-Caston, Catherine A. Kelly, Annabel V.L. Fitzgerald, Gary A. Leeke, Mike Jenkins, Tim W. Overton,