| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2019442 | Prostaglandins & Other Lipid Mediators | 2015 | 8 Pages |
•Tyrosol and resveratrol inhibit PAF production induced by IL-1β in U-937 cells.•Tyrosol is more potent inhibitor than resveratrol.•PAF levels after compounds incubation are mainly regulated by biosynthetic enzymes.•Acetylation seems to retain inhibitory effect of tyrosol.•Specific resveratrol acetylated derivatives exhibited dual action.
Interleukin 1 beta (IL-1β) induced platelet activating factor (PAF) synthesis in U-937 cells through stimulation of acetyl-CoA:lysoPAF-acetyltransferase (lyso PAF-AT) at 3 h and DTT-independentCDP-choline-1-alkyl-2-acetyl-sn-glycerol cholinophosphotransferase (PAF-CPT) at 0.5 h.The aim of this study was to investigate the effect of tyrosol (T), resveratrol (R) and their acetylated derivatives(AcDs) which exhibit enhanced bioavailability, on PAF synthesis in U-937 after IL-1β stimulation.The specific activity of PAF enzymes and intracellular levels were measured in cell homogenates. T and R concentration capable of inducing 50% inhibition in IL-1β effect on lyso PAF-AT was 48 μΜ ± 11 and 157 μΜ ± 77, for PAF-CPT 246 μΜ ± 61 and 294 μΜ ± 102, respectively. The same order of concentration was also observed on inhibiting PAF levels produced by IL-1β. T was more potent inhibitor than R (p < 0.05). AcDs of T retain parent compound inhibitory activity, while in the case of R only two AcDs retain the activity. The observed inhibitory effect by T,R and their AcDs, may partly explain their already reported beneficial role.
