| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2019457 | Prostaglandins & Other Lipid Mediators | 2016 | 7 Pages |
•Pulmonary artery vasoconstriction in rabbits is produced by 15(S)-HETE and by HETE analog 1.•Response to 15(S)-HETE is greater and sex-specific when compared to HETE analog 1.•15(S)-HETE produces more potent stimulation of vascular smooth muscle cell proliferation when compared to HETE analog 1.•In human hormone-independent prostate carcinoma PC-3 cells, HETE analog 1 inhibits proliferation and migration.•HETE analog 1 is a stable compound that will be a useful tool in future eicosanoid research.
15(S)-Hydroxyeicosa-(5Z,8Z,11Z,13E)-tetraenoic acid (15(S)-HETE) is a metabolite of arachidonic acid that elicits a number of biological effects including vasoconstriction and angiogenesis. (5Z,11Z,15R)-15-Hydroxyeicosa-5,11-dien-13-ynoic acid (HETE analog 1) is a synthetic isomer of 15(S)-HETE that is much more stable to autoxidation. Using isometric recording of isolated pulmonary arteries from male and female rabbits, HETE analog 1 and 15(S)-HETE were found to elicit concentration-dependent contractions that were slightly greater in females compared to males. The maximal response in females was greater with 15(S)-HETE. HETE analog 1 and 15(S)-HETE increased [3H]-thymidine incorporation in vascular smooth muscle cells cultured from male rabbit pulmonary arteries; both the maximal response and potency were greater with 15(S)-HETE. In contrast, HETE analog 1 produced a concentration-dependent inhibition in proliferation and migration of human hormone-independent prostate carcinoma PC-3 cells. The protocol for synthesis of HETE analog 1 is reported. The stability of this substance and its similar biological profile to 15(S)-HETE support future studies in eicosanoid research.
