| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2019762 | Prostaglandins & Other Lipid Mediators | 2011 | 10 Pages |
Abstract
⺠We study interactive effects of 15-deoxy-Î12,14-prostaglandin J2 (15d-PGJ2) in preadipocytes. ⺠15d-PGJ2 interferes specifically the inducible synthesis of endogenous prostanoids. ⺠The inhibition is due to the suppression of NF-κB activation and cyclooxygenase-2 induction. ⺠The prostanoids synthesized by preadipocytes attenuate the subsequent adipogenesis program. ⺠15d-PGJ2 rescues the suppressive effects of endogenous prostanoids on the adipogenesis program.
Keywords
NF-κB15d-PGJ2phorbol 12-myristate 13-acetatecPGESPDTCPPARγPGFSmPGESPVDF15-Deoxy-Δ12,14-PGJ2FBScytosolic PGE synthaseIBMXCOXlipocalin-type PGD synthase15-deoxy-Δ12,14-Prostaglandin J23-isobutyl-1-methylxanthineBSAPMAL-PGDSAdipocytebovine serum albumincyclooxygenasesodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGEReverse transcriptaseELISAEnzyme-linked immunosorbent assaydifferentiation mediumpolyvinylidene difluoridefetal bovine serumCyclooxygenase-2Nuclear factor-kappa Bmaturation mediumGrowth mediumpolymerase chain reactionPCRprostaglandinpyrrolidinedithiocarbamateperoxisome proliferator-activated receptor γ
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Authors
Abu Asad Chowdhury, Mohammad Sharifur Rahman, Kohji Nishimura, Mitsuo Jisaka, Tsutomu Nagaya, Takahiro Ishikawa, Fumiaki Shono, Kazushige Yokota,