| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 20198 | Journal of Bioscience and Bioengineering | 2015 | 5 Pages |
•The modes of action of COS/KA on Gram-positive and Gram-negative bacteria were examined.•COS/KA increased the membrane permeability by adhering to cell membranes.•COS/KA resulted in leakage of cell inclusions (enzymes, DNA, proteins and ions).•Gram-positive bacteria were more sensitive than Gram-negative bacteria to COS/KA.•COS/KA has potential in food industry and biomedical sciences.
Our work here, for the first time, reported the antibacterial activity of kojic acid-grafted-chitosan oligosaccharides (COS/KA) against three gram-positive and three gram-negative bacteria. Integrity of cell membrane, outer membrane (OM) and inner membrane (IM) permeabilization assay, alkaline phosphatase (ALP) and glucose-6-phosphate dehydrogenase (G6PDH) assay, and SDS-PAGE assay techniques were used to investigate the interactions between COS/KA and bacterial membranes. The antibacterial activity of COS/KA was higher than those of unmodified COS. The electric conductivity of bacteria suspensions increased, followed by increasing of the units of average release for ALP and G6PDH. COS/KA can also rapidly increase the 1-N-phenylanphthylamine (NPN) uptake and the release of β-galactosidase via increasing the permeability of OM and IM in Escherichia coli. SDS-PAGE indicated the content of cellular soluble proteins decreased significantly in COS/KA-treated bacteria. Hence, COS/KA has potential in food industry and biomedical sciences.
