| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2019878 | Prostaglandins & Other Lipid Mediators | 2009 | 8 Pages |
We investigated role(s) of luteal group IVA phospholipase A2 (GIVA PLA2) in prostaglandin (PG) F2α-induced regression in pseudopregnant rats. Prostaglandin F2α (PGF2α) treatment of day 6 pseudopregnant rats stimulated luteal PLA2 activity, which was sensitive to inhibitors and associated with increased GIVA PLA2 immunoreactivity. Intra-bursal treatment with the enzyme inhibitor (AACOCF3) prior to PGF2α failed to prevent the initial decline in progesterone but induced subsequently a persistent rise that was significantly higher than that of vehicle-treated group. TUNEL-positive signals in luteal cells of control group were reduced by AACOCF3 treatment. TUNEL-positive reaction induced in luteal cells in vitro by combined cytokines and agonistic anti-Fas were both reduced by AACOCF3 and another inhibitor pyrrophenone. Overall data show that luteal GIVA PLA2 activity and expression increased following PGF2α administration and that acute chemical inhibition of this activity could reverse, at least partly, PGF2α-induced functional regression and prevent apoptosis induced by PGF2αin vivo and by cytokines in vitro.
