Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2020227 | Protein Expression and Purification | 2016 | 5 Pages |
Abstract
α-Galactosidase (α-PsGal) of the cold-adapted marine bacterium Pseudoalteromonas sp. KMM 701 was cloned into the pET-40b(+) vector to study its properties and to develop an effective method for modifying human B-erythrocytes into O-blood group. The use of heat-shock as a pre-induction treatment, IPTG concentration of 0.2 mM and post-induction cultivation at 18 °C for 20 h in the developed MX-medium allowed increasing the recombinant Escherichia coli Rosetta (DE3)/40Gal strain productivity up to 30 times and the total soluble α-PsGal yield up to 40 times.
Keywords
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Biochemistry
Authors
V.A. Golotin, L.A. Balabanova, Yu. A. Noskova, L.V. Slepchenko, I. Yu Bakunina, N.S. Vorobieva, N.A. Terenteva, V.A. Rasskazov,