Article ID Journal Published Year Pages File Type
2020274 Protein Expression and Purification 2015 7 Pages PDF
Abstract
The kinetic properties were calculated: the recombinant HvCy-G6PDH showed KMs and KINADPH comparable to those observed for the enzyme purified from barley roots; moreover, the analysis of NADPH inhibition suggested a competitive mechanism. Therefore, this enzyme could be utilised for the structural and regulatory characterization of this isoform in higher plants.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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Expression and characterization of a cytosolic glucose 6 phosphate dehydrogenase isoform from barley (Hordeum vulgare) roots
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