Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2020304 | Protein Expression and Purification | 2015 | 5 Pages |
Abstract
In this study we expressed the extra-membrane domain of human CD90 (exCD90) antigen (Gln15-Cys130) in E. coli expression host cells. The epitope integrity of purified recombinant antigen was confirmed by antibody-antigen interaction using 5E10 anti-CD90 monoclonal antibody and binding study through ELISA and florescent staining of CD90+ cells in a flow cytometry experiment.
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Authors
Narges Yousefi-Rad, Mohammad Ali Shokrgozar, Mahdi Behdani, Shima Moradi-Kalbolandi, Mahdieh Motamedi-Rad, Mahdi Habibi-Anbouhi,