Article ID Journal Published Year Pages File Type
2020304 Protein Expression and Purification 2015 5 Pages PDF
Abstract
In this study we expressed the extra-membrane domain of human CD90 (exCD90) antigen (Gln15-Cys130) in E. coli expression host cells. The epitope integrity of purified recombinant antigen was confirmed by antibody-antigen interaction using 5E10 anti-CD90 monoclonal antibody and binding study through ELISA and florescent staining of CD90+ cells in a flow cytometry experiment.
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Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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