Article ID Journal Published Year Pages File Type
2020557 Protein Expression and Purification 2013 13 Pages PDF
Abstract
► Codon harmonization was used as a precautionary measure toward soluble JNK1β1 overexpression. ► JNK1β1 and ATF2 were purified in high yields to >99% purity using GST-tags. ► JNK1β1 was activated by reconstituting the MEKK1 → MKK4 → JNK MAPK pathway in vitro. ► Phosphorylated JNK1β1 had high specific activity toward its substrate, ATF2.
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