Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2020557 | Protein Expression and Purification | 2013 | 13 Pages |
Abstract
⺠Codon harmonization was used as a precautionary measure toward soluble JNK1β1 overexpression. ⺠JNK1β1 and ATF2 were purified in high yields to >99% purity using GST-tags. ⺠JNK1β1 was activated by reconstituting the MEKK1 â MKK4 â JNK MAPK pathway in vitro. ⺠Phosphorylated JNK1β1 had high specific activity toward its substrate, ATF2.
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Authors
Gavin R. Owen, Ikechukwu Achilonu, Heini W. Dirr,