| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2020640 | Protein Expression and Purification | 2012 | 10 Pages |
Abstract
⺠A protocol was developed to prepare pure isotopically labeled human Gαi1 for NMR. ⺠N-terminally truncated Gαi1 (with unstructured residues removed) was also prepared. ⺠Success depended on vector choice, low induction temperature and media supplements. ⺠NMR spectra recorded at 35 °C had better peak definition than at lower temperatures. ⺠Spectra provided preliminary evidence for widespread intramolecular motions in Gαi1.
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Authors
Jan Maly, Karin A. Crowhurst,