Article ID Journal Published Year Pages File Type
2020641 Protein Expression and Purification 2012 5 Pages PDF
Abstract
► The deacetylase from Pyrococcus horikoshii (Ph-Dac) was overexpressed as inclusion bodies in Escherichia coli. ► Ph-Dac was refolded as the bioactive homohexamer form. ► Ph-Dac exhibited specific deacetylase activity toward (GlcNAc)2.
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Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
Preview
Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii
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