| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2020662 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠Expression of recombinant Omp28 protein of Brucella melitensis in pET28a and pQE30UA vectors. ⺠Use of 1% Triton X-100 and 20 mM β-mercaptoethanol in buffers for single step purification. ⺠The modified purification protocol yielded 3.6-fold higher rOmp28 protein. ⺠The modified procedure may be useful for purification of proteins from inclusion bodies.
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Authors
Ashu Kumar, Sapana Tiwari, Duraipandian Thavaselvam, Kannusamy Sathyaseelan, Archana Prakash, Anita Barua, Sonia Arora, M. Kameswara Rao,