| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2020668 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠The refolding condition of α-amylase from Bacillus megaterium WHO was optimized by RSM. ⺠20% (v/v) glycerol, 25 mM Ca2+ and 0.3 (mg/ml) protein is the most efficient condition for refolding. ⺠Km and kcat value of refolded α-amylase with optimal conditions was much closer to soluble enzyme. ⺠DLS analysis indicated that optimized condition of refolding has generated more compact structure.
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Authors
Parisa Nasrollahi, Khosro Khajeh, Neda Akbari,