| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2020672 | Protein Expression and Purification | 2012 | 7 Pages |
Abstract
⺠High level expression of AXE1 in Pichia pastoris was achieved by codon and culture optimization. ⺠Strategy yielded a 246-fold increase in AXE1 levels compared to levels in Volvariella volvacea. ⺠N-linked glycosylation played an essential role in AXE1 secretion. ⺠N-glycosylation played little if any role in the catalytic activity of AXE1 or in enzyme stability.
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Authors
Bin Tian, Yan Chen, Shaojun Ding,