| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2020696 | Protein Expression and Purification | 2012 | 6 Pages |
Abstract
⺠Murine Igκ secretory signal drives secretion of fusion GalNAc-T2 in insect system. ⺠Secreted soluble human GalNAc-T2 was enzymatically active. ⺠The yield in Sf9 cells was 2.5 mg of GalNAc-T2 protein per 1 L culture. ⺠Recombinant GalNAc-T2 attaches GalNAc to all possible O-glycosylation sites in IgA1.
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Authors
Milada Horynová, Kazuo Takahashi, Stacy Hall, Matthew B. Renfrow, Jan Novak, Milan RaÅ¡ka,