Optimization of TNF-α overexpression in Escherichia coli using response surface methodology: Purification of the protein and oligomerization studies

Tumor necrosis factor-α (TNF-α) is responsible for many autoimmune disorders including rheumatoid arthritis, psoriasis, Chron’s disease, stroke, and atherosclerosis. Thus, inhibition of TNF-α is a major challenge in drug discovery. However, a sufficient amount of purified protein is needed for the in vitro screening of potential TNF-α inhibitors. In this work, induction conditions for the production of human TNF-α fusion protein in a soluble form by recombinant Escherichia coli BL21(DE3) pLysS were optimized using response surface methodology based on the central composite design. The induction conditions included cell density prior induction (OD600nm), post-induction temperature, IPTG concentration and post-induction time. Statistical analysis of the results revealed that all variables and their interactions had significant impact on production of soluble TNF-α. An 11% increase of TNF-α production was achieved after determination of the optimum induction conditions: OD600nm prior induction 0.55, a post induction temperature of 25 °C, an IPTG concentration of 1 mM and a post-induction time of 4 h. We have also studied TNF-α oligomerization, the major property of this protein, and a Kd value of 0.26 nM for protein dimerization was determined. The concentration of where protein trimerization occurred was also detected. However, we failed to determine a reliable Kd value for protein trimerization probably due to the complexibility of our model.

► We optimized TNF-α production in a soluble form using response surface methodology. ► Optimized factors were cell density, IPTG concentration, post-induction temperature and time. ► Protein yield increased approximately 11% after optimization. ► A kinetic study of TNF-α revealed a Kd of 0.26 nM for protein dimerization.