| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2020753 | Protein Expression and Purification | 2011 | 7 Pages |
Abstract
⺠Efficient expression of small helical membrane proteins with a mature MBP fusion in E. coli membranes. ⺠Positively charged tags (not necessarily His tags) on the N-terminus enhances expression of small helical membrane proteins. ⺠The mMBP fusion works best for helical membrane proteins having ⩽3 transmembrane helices and <20 kDa molecular weight. ⺠Frequently, one-step purification can be achieved using Ni2+-NTA chromatography following cleavage of the fusion protein.
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Authors
Jian Hu, Huajun Qin, Fei Philip Gao, Timothy A. Cross,