Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2020823 | Protein Expression and Purification | 2011 | 4 Pages |
Abstract
The cofactor heme (Fe-protoporphyrin IX) plays many important roles in biology. Identification of novel proteins for the transport, chaperoning and delivery of heme in cells is of widespread interest. Here, we describe the use of heme conjugated magnetic beads for the isolation of heme-binding proteins from complex protein mixtures. The reagent is straightforward to use, sensitive and specific.
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Authors
Rhian Jones, James W.A. Allen,