Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2021004 | Protein Expression and Purification | 2010 | 9 Pages |
Abstract
Human DNA polymerase ν (Pol ν) is a conserved family A DNA polymerase of uncertain biological function. Physical and biochemical characterization aimed at understanding Pol ν function is hindered by the fact that, when over-expressed in Escherichia coli, Pol ν is largely insoluble, and the small amount of soluble protein is difficult to purify. Here we describe the use of high hydrostatic pressure to refold Pol ν from inclusion bodies, in soluble and active form. The refolded Pol ν has properties comparable to those of the small amount of Pol ν that was purified from the soluble fraction. The approach described here may be applicable to other DNA polymerases that are expressed as insoluble inclusion bodies in E. coli.
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Biochemistry
Authors
Mercedes E. Arana, Gary K. Powell, Lori L. Edwards, Thomas A. Kunkel, Robert M. Petrovich,