Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2021028 | Protein Expression and Purification | 2010 | 5 Pages |
Abstract
Here we describe a procedure for the production in Pichia pastoris of Bv8 and 3 mutants: W24A-Bv8, in which the tryptophan in position 24 is substituted by alanine, the double mutant M1-W24A-Bv8, that contains an additional methionine at the N-terminus and Bv8-TyrTyr that includes two additional tyrosines at the C-terminus. The results evidence a relevant role of tryptophan 24 in Bv8-PKRs interaction.
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Authors
Rossella Miele, Roberta Lattanzi, Maria Carmela Bonaccorsi di Patti, Alessandro Paiardini, Lucia Negri, Donatella Barra,