Article ID Journal Published Year Pages File Type
2021028 Protein Expression and Purification 2010 5 Pages PDF
Abstract
Here we describe a procedure for the production in Pichia pastoris of Bv8 and 3 mutants: W24A-Bv8, in which the tryptophan in position 24 is substituted by alanine, the double mutant M1-W24A-Bv8, that contains an additional methionine at the N-terminus and Bv8-TyrTyr that includes two additional tyrosines at the C-terminus. The results evidence a relevant role of tryptophan 24 in Bv8-PKRs interaction.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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