Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2021144 | Protein Expression and Purification | 2010 | 5 Pages |
Abstract
Using published plasmid vectors containing the bgaB gene encoding a heat-stable β-galactosidase, we have been unable to fuse strong promoters to this reporter gene. In addition, we could not analyze the promoter strength by a plate assay. Therefore, we constructed an Escherichia coli–Bacillus subtilis shuttle vector to allow the cloning of strong promoters and their rapid analysis in B. subtilis by plating on X-Gal plates in the presence of the inducer IPTG. We show that the blue color of the colonies reflects the strength of the promoters, which was verified by measuring the β-galactosidase activities.
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Authors
Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann,