Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2021266 | Protein Expression and Purification | 2010 | 8 Pages |
Abstract
Islet Neogenesis Associated Protein (INGAP) is implicated in pancreatic islet neogenesis. INGAP peptide, a pentadecapeptide comprising amino acids 104–118, reverses diabetes in rodents and improves glucose homeostasis in patients with diabetes. The mechanism of INGAP action is unknown, but such studies would benefit from the availability of the full-length recombinant protein (rINGAP).Here we report the production of rINGAP from 293-SF cells following lentiviral transduction, and its characterization by MALDI-TOF and Q-TOF Mass Spectrometry, and HPLC.Importantly, we show that rINGAP exhibits 100× the bioactivity of INGAP peptide on a molar basis in an in vitro assay of human islet regeneration.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Béatrice Assouline-Thomas, Amélie Pilotte, Maria Petropavlovskaia, Julia Makhlin, Jieping Ding, David McLeod, Stephen Hanley, Bernard Massie, Lawrence Rosenberg,