Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2021423 | Protein Expression and Purification | 2009 | 5 Pages |
Abstract
Decay accelerating factor (DAF) is a very potent complement regulatory protein which holds promise for clinical usage. Here we report on an improved procedure for refolding both rat and human DAF over-expressed in Escherichia coli. It was shown that 50–70% of the inclusion body could be refolded to soluble active protein. This method excludes the use of l-arginine, which is expensive, and can be used to prepare a large quantity of recombinant DAF for therapeutic studies.
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Authors
Jiang Xu, Yongliang Hu, Meiliang Gong, Chenjing Sun, Zhikai Xu, Zhuyi Li, Xingan Wu,