Article ID Journal Published Year Pages File Type
2021423 Protein Expression and Purification 2009 5 Pages PDF
Abstract

Decay accelerating factor (DAF) is a very potent complement regulatory protein which holds promise for clinical usage. Here we report on an improved procedure for refolding both rat and human DAF over-expressed in Escherichia coli. It was shown that 50–70% of the inclusion body could be refolded to soluble active protein. This method excludes the use of l-arginine, which is expensive, and can be used to prepare a large quantity of recombinant DAF for therapeutic studies.

Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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