Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2021503 | Protein Expression and Purification | 2009 | 7 Pages |
Abstract
Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent indoor airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 was cloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This allergen was purified by immobilized Ni2+-affinity chromatography. The purified Pen b 26 was characterized by immunological, biochemical and biophysical methods. C-His6 tagged Pen b 26 produced several fold greater yield than N-His6 tagged Pen b 26. The affinity of C-His6 tagged Pen b 26 for the specific antibody was also 2.75 times higher than N-His6 tagged Pen b 26.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
M. Serdal Sevinc, Veena Kumar, Makonnen Abebe, Susantha Mohottalage, Premkumari Kumarathasan, Renaud Vincent, Hari M. Vijay,