Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2022441 | Regulatory Peptides | 2013 | 4 Pages |
Abstract
The synthetic peptide octarphin (TPLVTLFK) corresponding to the sequence 12-19 of β-endorphin, a selective agonist of nonopioid β-endorphin receptor, was labeled with tritium to specific activity of 29 Ci/mmol. The analysis of [3H]octarphin binding to rat pituitary and adrenal cortex membranes revealed the existence of one type of binding sites (receptors): Kd 5.9 and 35.6 nM, respectively. Octarphin at concentrations of 1-1000 nРwas shown to inhibit the adenylate cyclase activity of rat adrenocortical membranes, while its intramuscular injection at doses of 10-100 μg/kg was found to reduce the secretion of corticosterone from the adrenals to the bloodstream. Thus, the nonopioid receptor of β-endorphin may be involved in the regulation of the activity of the pituitary and adrenal glands.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Yuliia N. Nekrasova, Yury A. Zolotarev, Elena V. Navolotskaya,