Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2023006 | Regulatory Peptides | 2009 | 5 Pages |
Abstract
Chemerin has recently been characterized as a novel adipokine playing a crucial role in adipocyte differentiation and insulin signalling. In the current study, the impact of insulin resistance-inducing and proinflammatory interleukin (IL)-1à on chemerin protein secretion and mRNA expression was determined in 3T3-L1 adipocytes. Interestingly, IL-1à significantly induced chemerin protein secretion almost 1.3-fold from 5.89 ng/ml (basal) to 7.52 ng/ml. Furthermore, chemerin mRNA synthesis was significantly stimulated by IL-1à in a dose-dependent fashion with 1.5-fold induction seen at IL-1à concentrations as low as 0.07 ng/ml and maximal 2.6-fold upregulation found at 2 ng/ml effector. Induction of chemerin mRNA by IL-1à was time-dependent in both 3T3-L1 adipocytes and brown fat cells. Signalling studies suggested that Janus kinase 2, nuclear factor κ B, p44/42 mitogen-activated protein kinase, and phosphatidylinositol 3-kinase are involved in IL-1Ã-induced chemerin mRNA expression. Furthermore, recombinant chemerin downregulated insulin-stimulated glucose uptake. Taken together, we show that chemerin is upregulated in fat cells by IL-1à and might modulate the effects of IL-1à on adipocyte metabolism and insulin sensitivity.
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Authors
Susan Kralisch, Sebastian Weise, Grit Sommer, Jana Lipfert, Ulrike Lossner, Matthias Bluher, Michael Stumvoll, Mathias Fasshauer,