Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2023237 | Regulatory Peptides | 2008 | 7 Pages |
Abstract
We investigated whether ghrelin depletion (by gastrectomy surgery) and/or treatment/replacement with the gastric hormone ghrelin alters the expression of key hypothalamic genes involved in energy balance, in a manner consistent with ghrelin's pro-obesity effects. At 2Â weeks after surgery mice were treated with ghrelin (12Â nmol/mouse/day, sc) or vehicle for 8Â weeks. Gastrectomy had little effect on the expression of these genes, with the exception of NPY mRNA in the arcuate nucleus that was increased. Ghrelin treatment (to gastrectomized and sham mice) increased the mRNA expression of orexigenic peptides NPY and AgRP while decreasing mRNA expression of the anorexigenic peptide POMC. Two weeks gavage treatment with the ghrelin mimetic, MK-0677, to rats increased NPY and POMC mRNA in the arcuate nucleus and MCH mRNA in the lateral hypothalamus. Thus, while predicted pro-obesity ghrelin signalling pathways were activated by ghrelin and ghrelin mimetics, these were largely unaffected by gastrectomy.
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Authors
Emil Egecioglu, Björn Stenström, Scarlett B. Pinnock, Loraine Y.C. Tung, Charlotta Dornonville de la Cour, Andreas Lindqvist, Rolf HÃ¥kanson, Unni Syversen, Duan Chen, Suzanne L. Dickson,