Angiotensin II and Aldosterone stimulating NF-κB and AP-1 activation in hepatic fibrosis of rat

Background/aimsIntrahepatic renin–angiotensin–aldosterone system (RAAS) plays a key role in the fibrogenesis of liver. However, the signal transduction mechanism underlying effects of Angiotensin II (Ang II) and Aldosterone (Aldo) on Nuclear Factor-κB (NF-κB) and active protein-1 (AP-1) pathway in hepatic fibrogenesis remains to be fully elucidated. The present study aims to investigate the signal transduction mechanism underlying effects of Ang II and Aldo on NF-κB and AP-1 pathway during hepatic fibrogenesis.MethodsTo assess the effect of AECI and Angiotensin II type 1 receptor (AT-1 receptor) blocker on NF-κB activity in liver, a model of fibrosis was performed in rat. In vitro, hepatic stellate cells (HSCs)-T6 cells were preincubated for 1 h or not with U0126, a specific inhibitor of extracellular signal regulated kinase (ERK), irbesartan, and N-acetylcysteine prior to exposure to Ang II or Aldo for the indicated times. DNA binding activity of NF-κB and AP-1 were analyzed by Electrophoretic mobility shift assay (EMSA). Western blot was used to detect expression of IκBα and Phospho-P42/44. RT-PCR was used to detect the expressions of tumor necrosis factor α (TNFα) mRNA and α1 (I) procollagen mRNA.ResultsAECI and AT-1 receptor blocker exert anti-fibrosis effect through inhibiting NF-κB activation in liver. Ang II and Aldo increase HSCs NF-κB activity and NF-κB target gene-TNFα expression by inhibiting IκBα expression in a redox-sensitive manner. Ang II and Aldo also markedly increase HSCs AP-1 activity and AP-1 target gene-α1 (I) procollagen mRNA expression via ERK1/2 pathway in a redox-sensitive manner.ConclusionsThese results show that stimulation of NF-κB and AP-1 pathway mediate hepatic fibrogenesis induced by intrahepatic RAAS.