| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2023569 | Regulatory Peptides | 2006 | 6 Pages |
ObjectiveWe analysed the effect of aldosterone on calcitonin gene-related peptide (CGRP) mediated vasodilation in noradrenaline precontracted endothelium denuded mesenteric arteries segments from Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) and the effect of aldosterone on calcitonin receptor-like receptor (CL receptor) and receptor activity modifying protein 1 (RAMP1) expression in endothelium-denuded mesenteric arteries from SHR rats.ResultsCGRP 0.1 nM–0.1 μM induced a concentration-dependent relaxation that was enhanced by aldosterone 1 μM in SHR only. Incubation with RU 486 10 μM significantly reduced the enhancement of CGRP-relaxation produced by aldosterone in SHR. CL receptor expression was not modified in either strain, while RAMP1 expression was enhanced in SHR by aldosterone 1 μM 120 min and 0.1 μM 120 min. This up-regulation of RAMP1 was prevented by RU 486 10 μM.ConclusionsAldosterone, through glucocorticoid receptor activation, increases the vasodilatory effect of CGRP in SHR mesenteric arteries, which seems to be mediated by increased RAMP1 expression.
