| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 20276 | Journal of Bioscience and Bioengineering | 2015 | 4 Pages |
Abstract
•Rapid and high-throughput detection for antibody glycosylation.•The detection is based on chemiluminescent-bead-based lectin binding.•This method is applicable to evaluation of glycosylation dynamics in cell culture.
We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Masayoshi Onitsuka, Takeshi Omasa,