Tandem mass spectrometric characterization of bile acids and steroid conjugates based on low-energy collision-induced dissociation

- Glycine and taurine conjugates gave specific product ions at m/z 74 and 124.
- Sulfates gave a specific product ion at m/z 97 with some neutral losses.
- Fragmentation of glucuronides differed from each other by bond types.
- Glucosides and galactosides gave a neutral loss of 162 Da.
- Conjugate groups affect each other's fragmentation.