New cytotoxic steroids from the leaves of Clerodendrum trichotomum

•Five new steroids were isolated and elucidated by extensive NMR experiments.•Steroidal side chain with two double bonds at C-22 and C-25 are rare in nature.•The steroids were evaluated for cytotoxicity against HeLa cell line.•Steroids 2 and 5 exhibited moderate cytotoxicity.

A phytochemical investigation of the leaves of Clerodendrum trichotomum led to the isolation of five new (2–6) and two known (1 and 7) steroids, whose structures and relative configurations were elucidated by comprehensive spectroscopic analysis and by comparison of their NMR data with those of related compounds. Steroids 2 and 5 exhibited moderate cytotoxicity in vitro against HeLa cell line.

Graphical abstractFive new (2–6) and two known (1 and 7) steroids were isolated from the leaves of Clerodendrum trichotomum. Steroids 2 and 5 exhibited moderate cytotoxicity in vitro against HeLa cell line.Figure optionsDownload full-size imageDownload as PowerPoint slide