| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2029299 | Steroids | 2011 | 9 Pages |
Abstract
⺠We synthesized the 3- and 21-monosulfates of pentadeuterated 5β-tetrahydrocorticosteroids. ⺠Exchange reaction of the 5β-3-ketosteroids with NaOD in CH3OD followed by NaBD4 was employed. ⺠Sulfation of C-3 and C-21 hydroxy groups was attained by treatment with SO3-TMA complex. ⺠The isotopic purification of the labeled compounds as [2H5]-form were more than 95 atom% D. ⺠The labeled compounds can be used as internal standards in LC/MS assays.
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Biochemistry
![Synthesis of 3- and 21-monosulfates of [2,2,3β,4,4-2H5]-tetrahydrocorticosteroids in the 5β-series as internal standards for mass spectrometry](/preview/png/2029299.png "First Page Preview: Synthesis of 3- and 21-monosulfates of [2,2,3β,4,4-2H5]-tetrahydrocorticosteroids in the 5β-series as internal standards for mass spectrometry")
Authors
Shigeo Ikegawa, Kaori Nagae, Takayuki Mabuchi, Rika Okihara, Maki Hasegawa, Toshie Minematsu, Takashi Iida, Kuniko Mitamura,