Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
20304 | Journal of Bioscience and Bioengineering | 2015 | 4 Pages |
Abstract
We truncated the short arm of chromosome 3 to delete the aflatoxin biosynthesis gene homolog cluster using telomeric repeats in Aspergillus oryzae. The predicted deletion was confirmed by Southern blot analyses. This telomere-mediated chromosomal truncation method enables the development of an artificial chromosome in A. oryzae.
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Authors
Sawaki Tada, Hikaru Ohkuchi, Mayumi Matsushita-Morita, Ikuyo Furukawa, Ryota Hattori, Satoshi Suzuki, Yutaka Kashiwagi, Ken-Ichi Kusumoto,