Structural Basis for the PufX-Mediated Dimerization of Bacterial Photosynthetic Core Complexes

SummaryIn Rhodobacter (Rba.) sphaeroides, the subunit PufX is involved in the dimeric organization of the core complex. Here, we report the 3D reconstruction at 12 Å by cryoelectron microscopy of the core complex of Rba. veldkampii, a complex of ∼300 kDa without symmetry. The core complex is monomeric and constituted by a light-harvesting complex 1 (LH1) ring surrounding a uniquely oriented reaction center (RC). The LH1 consists of 15 resolved α/β heterodimers and is interrupted. Within the opening, PufX polypeptide is assigned at a position facing the QB site of the RC. This core complex is different from a dissociated dimer of the core complex of Rba. sphaeroides revealing that PufX in Rba. veldkampii is unable to dimerize. The absence in PufX of Rba. veldkampii of a G31XXXG35 dimerization motif highlights the transmembrane interactions between PufX subunits involved in the dimerization of the core complexes of Rhodobacter species.