Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel

We fabricated a three-dimensional multilayered blood vessel model using human cells and high-strength PEG hydrogel. The hydrogel tube was physically suitable for perfusion culture, and cells were cultured on the hydrogel surface by binding with fibronectin. Using the layer-by-layer cell multilayered technique, we successfully constructed an artificial blood vessel.