| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 20759 | Journal of Bioscience and Bioengineering | 2013 | 4 Pages |
Abstract
Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Jing Zhang, Satoshi Yamaguchi, Hidehiko Hirakawa, Teruyuki Nagamune,