| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089657 | Journal of Microbiological Methods | 2016 | 4 Pages |
Abstract
A new multi-pulse electroporation system was evaluated to transform Staphylococcus aureus. Compared to the conventional electroporation system, it yielded high transformation efficiency to obtain more than 3.9 Ã 105 S. aureus RN4220 transformed cells/1 μg plasmid DNA using a single electroporation by manipulating the poring pulse and transfer pulse.
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Authors
Junzo Hisatsune, Yusuke Sato'o, Liansheng Yu, Shoko Kutsuno, Yasuhiko Hayakawa, Motoyuki Sugai,