| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 2089672 | Journal of Microbiological Methods | 2016 | 5 Pages |
•Rapid and safe pretreatment procedure for MALDI-TOF yeast avoiding formic acid.•Performance of glass bead new extraction method for the accurate identification of pathogenic Candida species.•Creation and validation of yeast database library using clinical isolates.
In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling.
