Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay

•Production of Salmonella flagellar protein FlgK•Immunization of chickens with the FlgK protein•Use of FlgK antibodies from chicken sera to develop automated capillary immunoassay

Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.