Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry for species identification of Nonfermenting Gram-Negative Bacilli

•MALDI-TOF MS had a good performance in the identification of 29 genera of NFGNB.•MALDI-TOF MS is a useful tool for the rapid diagnosis of NFGNB including rare species.•MALDI-TOF MS database needs to be expanded and updated for better performance.

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) to identify 396 Nonfermenting Gram-Negative Bacilli clinical isolates was evaluated in comparison with conventional phenotypic tests and/or molecular methods.MALDI-TOF MS identified to species level 256 isolates and to genus or complex level 112 isolates. It identified 29 genera including uncommon species.